Bovine blastocysts with developmental competence to term share similar expression of developmentally important genes although derived from different culture environments

Effect of Culture System on Developmental Competence, Cryosurviva and DNA-Fragmentation of In Vitro Bovine Blastocysts

P-47: Effect of Blastocoelic Fluid Reduction on Quality and Expression of Developmentally Important Genes in Mouse Blastocysts

Background Recent researches reveal that manual puncturing of the trophectoderm of blastocyst before vitrification, increase the quality of embryo. However, in any of these studies, the importance of blastocoelic fluid and its impact on the formation of three cell lines is not mentioned. Therefore, in the present study, the effect of blastocoelic fluid reduction before vitrification on survival...

Gene expression in bovine embryos derived from oocytes with different developmental competence collected at the defined follicular developmental stage.

The aim of this study was to compare the expression of selected genes in bovine embryos developed from oocytes with different developmental competence. Four oocyte populations were collected, separately either from small (2-5 mm) or medium (6-10 mm) follicles, in the growth/stagnation (G/S) or dominance/regression (D/R) stage of the first follicular wave. They were matured, fertilized and cultu...

Effect of Culture System on Developmental Competence, Cryosurvival and DNA-Fragmentation of In Vitro Bovine Blastocysts

BACKGROUND This study investigated the effect of two in vitro embryo culture systems (co-culture system versus cell-free sequential-media) on developmental competence, cryosurvival and DNA- fragmentation of in vitro developed bovine blastocysts. MATERIALS AND METHODS Bovine presumptive zygotes were cultured in Ménézo's B2 (B2) plus vero-cells or sequential synthetic oviductal fluid (SOF) for ...

effect of culture system on developmental competence, cryosurviva and dna-fragmentation of in vitro bovine blastocysts

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